Purification and Characterization of Azoreductase from B. licheniformis
نویسندگان
چکیده
Dye decolorizing bacteria was isolated and identified from textile effluent samples. The isolate was identified as Bacillus licheniformis by 16S rDNA sequencing. Azoreductase, an enzyme responsible for catalyzing the reductive cleavage of azo bonds was purified from B.licheniformis. The enzyme was purified by a combination of ammonium sulphate precipitation, ion exchange and affinity chromatography. The optimum pH and temperature of purified enzyme was found to be 7 and 20oC. The molecular mass of purified enzyme determined by SDS-PAGE was found to be 18 kDa.
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